Katherine "Kate" Kane and her twin sister, Elizabeth "Beth" Kane, were born into the wealthy Kane family in Gotham city. On their twelfth birthday, Kate and Beth were taken out to a restaurant by their mother where they were kidnapped and held for ransom. Their father, Jake Kane; a military colonel, launched a rescue attempt but only Kate survived the conflict; her mother and sister killed by the kidnappers.
Years later Kate attended the United States Military Academy only to be expelled, despite her excellent grades, due to her sexual orientation. Returning to Gotham she began to suffer depression over having no life direction, however a chance mugging-attempt resulted in her meeting Batman saw her inspired and she attempts to fight crime using her father's military equipment in secret.
Discovered by and further assisted by her father, Kate is sent on a two year training regime after which she returns to Gotham to find a Batsuit and secret bunker ready for her use.
- During the Batcave: Outer Caverns raid, the party will have to fight her, Nightwing, Robin, and Huntress as they become infected by the OMAC virus and become OMACs.
- If you choose Batman as a mentor the player will team up with Batwoman to fight Scarecrow in the Gotham City Sewers.
- Batwoman is located in the GCPD 9th Precinct next to the Booster Gold Kiosk, and offers medium-level missions to hero players.
- Batwoman is involved in the Intergang quest. She must be rescued by the heroes before she is sacrificed by Bruno Mannheim. They are assisted by Question.
- Batwoman is involved in the Intergang quest. She is being sacrificed by Bruno Mannheim as the villains attempt to steal the Crime Bible. Assisted by Deathstroke, they leave her for dead after taking the Crime Bible.
- Batwoman appears randomly as an opponent in the Police Station Tier 3 Duo.
- Batwoman (Katherine Kane) first appeared in 52 #7 (July 2006)
- Batwoman is voiced by Christina J. Moore
- Kate Kane lives in the R.H. Kane Building.
- Revealed during an encounter with Scarecrow, Batwoman seems to be afraid of lightning and thunder.
- Kate and Renee Montoya were once lovers back when Kate was just a socialite and Montoya was an officer in the GCPD.
- Kate was dismissed from military service due to the Don't ask, don't tell government policy that only allowed gays, lesbians, and bisexuals to serve in the military if they kept their sexual orientation secret and the military did not learn of their sexual orientation. This policy has since be repealed and gays, lesbians, and bisexuals can now serve openly in the United States Armed Forces.