Poison Ivy is Gotham City's resident eco-terrorist. A unique human/plant hybrid, she cares only for her beloved plants and their ultimate dominion over the planet. Poison Ivy's once-human body has been chemically altered and can create any number of pheromones and toxins to entice or destroy.
Her unique bond with plants means she can swiftly generate new breeds or plant explosive seed pods on her enemies.
Pale, gangly and unremarkable, Pamela Isley was a shy botanist when she went to work for famed scientist Dr. Jason Woodrue. Hoping to create a human/plant hybrid, Woodrue experimented on Isley, creating the beautiful-but-deadly Poison Ivy. Now a physical beauty with chlorophyll instead of blood and capable of exuding various pheromones and natural toxins, Ivy began a criminal career to fund her cause as a champion for the world's diminishing flora species.
Her body can be seen lying to the right and just a short distance past Green Arrow's as the "Who do you Trust" trailer opens.
- She is one of the bosses in Arkham Asylum (Alert). She, Scarecrow, and Mr. Freeze have taken over Arkham Asylum and Heroes and Villains must fight them.
- She is also the center of attention of the Spring Seasonal event where Heroes must stop her plants from taking over Gotham while Villains try to help her. Ivy intends to tap into The Green in order to spread her "babies" across the planet.
- Poison Ivy has released her legions of plants to spread across the Giordano Botanical Gardens area. Robin has gone ahead to investigate. You must clear the area of her plant pods and locate Robin inside Poison Ivy's lair.
- During the Spring Seasonal Event, she gives villain players their quest. She's located in The Pit.
- Poison Ivy first appeared in Batman #181 (June 1966).
- Poison Ivy is voiced by Cyndi Williams.
- In the game's cinematic trailer, Poison Ivy is seen dead; lying in the rubble of Metropolis.
- Pamela Isley, mousy scientist, still lurks in Poison Ivy's psyche. So the new and improved Ivy enjoys her new feminine wiles to the utmost, luring men in only to use and destroy them.
- Ivy's best friend is the Joker's sidekick, Harley Quinn.
- Ivy's skin is green due to the deadly toxins that flow though her body. As such she is immune to most toxins and possesses a "poison kiss" which can paralyze and kill her foes.
- When fighting in The Greenhouse, her stance is that of Martial Arts. Yet in the Arkham Asylum (Alert) her stance is that of Hand Blaster.